MSAP marker based DNA methylation study in Salicornia brachiata DREB2A transgenic tobacco
نویسندگان
چکیده
منابع مشابه
Overexpression of SbSI-1, A Nuclear Protein from Salicornia brachiata Confers Drought and Salt Stress Tolerance and Maintains Photosynthetic Efficiency in Transgenic Tobacco
A novel Salicornia brachiata Salt Inducible (SbSI-1) gene was isolated and overexpressed in tobacco for in planta functional validation subjected to drought and salt stress. SbSI-1 is a nuclear protein. The transgenic tobacco overexpressing SbSI-1 gene exhibited better seed germination, growth performances, pigment contents, cell viability, starch accumulation, and tolerance index under drought...
متن کاملStudy of DNA methylation by tobacco-specific N-nitrosamines.
An enzyme-linked immunosorbent assay (BA-ELISA) involving use of biotin-labeled anti-rabbit IgG and avidin-labeled horseradish peroxidase was developed for the measurement of O6-methyl-2'-deoxyguanosine (O6-MedGuo). Up to 5 micrograms of methylated DNA was enzymatically hydrolyzed, and the extent of inhibition of binding of immobilized O6-MedGuo-bovine serum albumin to rabbit anti-O6-MedGuo was...
متن کاملMSAP markers and global cytosine methylation in plants
17 Methylation of DNA cytosines affects whether transposons are silenced and genes are 18 expressed, and is a major epigenetic mechanism whereby plants respond to 19 environmental change. Analyses of Methylation-Sensitive Amplification Polymorphism 20 (MS-AFLP or MSAP) have been often used to assess methyl-cytosine changes in 21 response to stress treatments and, more recently, in ecological st...
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The SbASR-1 gene, cloned from a halophyte Salicornia brachiata, encodes a plant-specific hydrophilic and stress responsive protein. The genome of S. brachiata has two paralogs of the SbASR-1 gene (2549 bp), which is comprised of a single intron of 1611 bp, the largest intron of the abscisic acid stress ripening [ASR] gene family yet reported. In silico analysis of the 843-bp putative promoter r...
متن کاملStudies on the Phytochemistry, Spectroscopic Characterization and Screening for Anti-mitotic Efficacy of Salicornia Brachiata Roxb
Salicorniabrachiata is a euhalopytic plant belonging to the family Chenopodiaceae. The present study investigates the phytochemistry, characterization and antimitotic activity of ethanolic extract of S.brachiata.Plants popularly known as Sea asparagus are cooked and eaten or pickled. It is also a good fodder for cattle, sheep and goat. Plant material is also used as raw material in paper and bo...
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ژورنال
عنوان ژورنال: Plant Gene
سال: 2016
ISSN: 2352-4073
DOI: 10.1016/j.plgene.2016.04.001